Recombinant DENV Envelope Protein, His-tagged

• Cat.No.: Envelope-513D
• Product Overview: Recombinant DENV Envelope Protein(ACF49259.1)(Val580-Lys680), fused with His tag, was expressed in HEK293.

Specification

• Species: DENV
• Source: HEK293
• Tag: His
• Protein Length: Val580-Lys680
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant Dengue virus (DENV)(type 1, strain US/Hawaii/1944) E consists of 112 amino acids and predicts a molecular mass of 12.4 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.