Recombinant Dengue virus type 3 (strain China/80-2/1980) NS1 protein, His-tagged

• Cat.No.: NS1-12D
• Product Overview: Recombinant Dengue virus type 3 (strain China/80-2/1980) NS1 protein(Met775-Ala1125), fused with His tag, was expressed in HEK293.

Specification

• Species: DENV3
• Source: HEK293
• Tag: His
• Protein Length: Met775-Ala1125
• Description: Dengue virus (DENV) is a single-stranded positive-sense RNA virus that causes dengue fever, belonging to the Flaviviridae family. A total of four antigenically distinct serotypes (DENV1, DENV2, DENV3 and DENV4) have been identified with different manifestations of clinical symptoms despite sharing 65% similarities. DENV non-structural protein 1 (NS1), one of the 7 non-structural proteins in DENV, is a highly conserved 48-kDa glycoprotein with serotype-specific epitopes that enable differentiation of DENV serotypes. NS1 is the only protein that is continuously secreted by infected host cells. NS1 is secreted in a hexamer form, which is composed of three dimers with a detergent-sensitive hydrophobic central cavity that carries a cargo of ～70 lipid molecules. The NS1 can be detected in the serum of patients from the onset of symptoms, which serves as a more useful diagnostic marker for the early detection of DENV. Furthermore, DENV NS1 plays a pathogenic role in vascular leakage.
• Form: Phosphate Buffered Saline,5%mannital,0.05% Tween20, pH7.4
• Molecular Mass: 39.71 kDa
• Purity: >95% pure (SDS-PAGE).
• Storage: For long term storage, the product should be stored at -20°C or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.