Recombinant Dengue virus (DENV)(type 3, strain Philippines/H87/1956) E protein(Phe247-Ser675), His-tagged

• Cat.No.: E-2438D
• Product Overview: Recombinant Dengue virus (DENV)(type 3, strain Philippines/H87/1956) Envelope Protein (AAA99437.1) (Phe247-Ser675) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: DENV
• Source: HEK293
• Tag: His
• Protein Length: Phe247-Ser675
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Dengue virus (DENV)(type 3, strain Philippines/H87/1956) Envelope Protein comprises 442 amino acids and has a calculated molecular mass of 48.79 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.