Recombinant Cynomolgus TNFSF13B protein, His-Avi-Flag-tagged, Biotinylated

• Cat.No.: TNFSF13B-002C
• Product Overview: Biotinylated Recombinant Cynomolgus TNFSF13B protein(A0A2K5V2X4)(Thr141-Leu285), fused with N-terminal His, Avi and Flag tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&Flag&His
• Protein Length: Thr141-Leu285
• Tag: N-His-Avi,N-Flag
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Cynomolgus BAFF (Trimer), His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.