Recombinant Cynomolgus TNFRSF8 protein, His-tagged

• Cat.No.: TNFRSF8-046C
• Product Overview: Recombinant Cynomolgus TNFRSF8 protein(A0A2K5VW07)(Ala27-Pro394) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: C-His
• Protein length: Ala27-Pro394
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus CD30, His Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-CD30 Antibody, hFc Tag with the EC50 of 6.0 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 39.86 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: >95% as determined by Bis-Tris PAGE
  >95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3 months after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.