Recombinant Cynomolgus SIRPA protein, His-tagged
Cat.No. : | SIRPA-343C |
Product Overview : | Recombinant Cynomolgus SIRPA protein(NP_001271679.1)(Glu31-Arg370), fused with C-terminal His tag, was expressed in HEK293. |
Availability | March 14, 2025 |
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Species : | Cynomolgus |
Source : | HEK293 |
Tag : | His |
Protein Length : | Glu31-Arg370 |
Tag : | C-His |
Form : | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Cynomolgus SIRP alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. |
Molecular Mass : | The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
Endotoxin : | Less than 1EU per μg by the LAL method. |
Purity : | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Storage : | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Reconstitution : | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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