Recombinant Cynomolgus SEMA4D protein, His-tagged

• Cat.No.: SEMA4D-211C
• Product Overview: Recombinant Cynomolgus SEMA4D protein(A0A2K5TZC9)(Phe24-Arg734), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: C-His
• Protein length: Phe24-Arg734
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus Semaphorin 4D, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 11.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 80.1 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.