Recombinant Cynomolgus/Rhesus MET protein(Met1-Thr932)

• Cat.No.: MET-1176C
• Product Overview: Recombinant Cynomolgus / Rhesus MET (NP_001162100.1) (Met1-Thr932) was expressed in HEK293 with five amino acids (DDDDK) the C-terminus. Cynomolgus and Rhesus MET sequences are identical.

Specification

• Source: HEK293
• Species: Cynomolgus/Rhesus
• Tag: Non
• Protein length: Met1-Thr932
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Cynomolgus MET at 10 μg/ml (100 μl/well) can bind biotinylated Cynomolgus HGF, The EC50 of biotinylated Cynomolgus HGF is 0.17-0.41 μg/ml.
• Molecular Mass: The recombinant cynomolgus / rhesus MET comprises 914 amino acids and has a calculated molecular mass of 102.3 KDa. The apparent molecular mass of it is approximately 73-93 KDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.