Recombinant Cynomolgus/Rhesus macaque CTLA4 protein, Fc-tagged

• Cat.No.: CTLA4-370C
• Product Overview: Recombinant Cynomolgus/Rhesus macaque CTLA4 protein (Ala37-Ser160), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).In the region Ala37-Ser160, the AA sequence of Cynomolgus and Rhesus macaque CTLA4 are homologus.

Specification

• Species: Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: Fc
• Protein Length: 124
• Description: CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 40.0 kDa. As a result of glycosylation, the protein migrates as 45-52 kDa under reducing (R) condition, and 90-105 kDa under non-reducing (NR) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CTLA4
• Official Symbol: CTLA4
• Synonyms: CTLA-4
• Gene ID: 705673
• mRNA Refseq: NM_001044739.1
• Protein Refseq: NP_001038204.1
• UniProt ID: Q9BDC4