Recombinant Cynomolgus NCR3LG1 protein, His-tagged

• Cat.No.: NCR3LG1-2942C
• Product Overview: Recombinant Cynomolgus NCR3LG1 protein(XP_005578557.1)(Asp25-Asp259), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Asp25-Asp259
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H6, His Tag with an affinity constant of 0.662 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 27.6 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.