Recombinant Cynomolgus MUSK Protein, Fc&Avi tagged, Biotin Labeled

• Cat.No.: MUSK-49C
• Product Overview: Recombinant Cynomolgus Monkey MUSK Protein (Leu24-Thr495) with Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: This gene encodes a muscle-specific tyrosine kinase receptor. The encoded protein may play a role in clustering of the acetylcholine receptor in the postsynaptic neuromuscular junction. Mutations in this gene have been associated with congenital myasthenic syndrome. Alternatively spliced transcript variants have been described.
• Source: CHO
• Species: Cynomolgus Monkey
• Tag: C-Fc
• Protein length: 24-495aa
• Conjugation/Label: Biotin
• Form: Disulfide-linked homodimer, Biotinylated via Avi-tag
• Bio-activity: Measured by its binding ability in a functional ELISA. In the presence of Recombinant Human Agrin, immobilized Recombinant Cynomolgus Monkey MuSK Fc Chimera at 0.5 μg/mL (100 μL/well) binds Recombinant Human LRP-4 with an ED50 of 10.0-80.0 ng/mL.
• Molecular Mass: 78 kDa
• Endotoxin: < 0.1 EU/μg by LAL.
• Purity: > 95 %, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• N-terminal Sequence Analysis: Leu24
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• References: 1. Burden, S.J. et al. (2013) CSH Perspectives Bio. 5(5).
  2. Hubbard, S.R. and Gnanasambandan, K. (2013) Biochem Biophys Acta. 1834(10):2166.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.

Gene Information

• Gene Name: MUSK muscle associated receptor tyrosine kinase [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: MUSK
• Synonyms: MUSK; muscle associated receptor tyrosine kinase; muscle, skeletal receptor tyrosine-protein kinase
• Gene ID: 102127677
• mRNA Refseq: XM_005581093
• Protein Refseq: XP_005581150
• UniProt ID: G7PRP5