Recombinant Cynomolgus MSLN protein, His-tagged, Biotinylated(Primary Amine Labeling)

• Cat.No.: MSLN-5732C
• Product Overview: Biotinylated Recombinant Cynomolgus MSLN protein(A0A2K5TW94-1)(Asp296-Gly580)(Primary Amine Labeling), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: C-His
• Protein length: Asp296-Gly580
• Conjugation/Label: Biotin; Primary Amine
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-MSLN Antibody, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Cynomolgus MSLN, His Tag with the EC50 of 44.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 33 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.