Recombinant Cynomolgus IL3 protein, His-tagged

• Cat.No.: IL3-1504C
• Product Overview: Recombinant Cynomolgus IL3 protein(G7P877)(Ala20-Gln143), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Cynomolgus
• Source: N-His
• Protein Length: Ala20-Gln143
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Cynomolgus IL-3 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-3, His Tag with an affinity constant of 1.30 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 15.13 kDa same as Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.