Recombinant Cynomolgus IGF1R protein, His-tagged

• Cat.No.: IGF1R-391H
• Product Overview: Recombinant Cynomolgus IGF1R protein (Glu31-Asn932), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 902
• Description: The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 104.9 kDa, 81.1 kDa, 23.8 kDa. The protein migrates as 45-50 kDa, 96-115 kDa and 120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IGF1R
• Official Symbol: IGF1R
• Synonyms: IGF1R; insulin-like growth factor 1 receptor; IGF-I receptor; insulin-like growth factor I receptor; JTK13; IGFIRC
• Gene ID: 708227
• mRNA Refseq: NM_001261352.1
• Protein Refseq: NP_001248281.1
• UniProt ID: I2CWY3