Recombinant Cynomolgus HJV protein, His-tagged

• Cat.No.: HJV-1394C
• Product Overview: Recombinant Cynomolgus HJV protein(XP_005542141.1)(Gln36-Asp401), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: C-His
• ProteinLength: Gln36-Asp401
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Cynomolgus RGM-C, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 40 kDa (mature)&26 kDa (C-terminus peptide)&14 kDa (N-terminus peptide). Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa&30-40 kDa&20-25 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.