Recombinant Cynomolgus CD40 protein, His-tagged

• Cat.No.: CD40-8823C
• Product Overview: Recombinant Cynomolgus CD40 protein(G7PG38)(Glu21-Arg193), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• ProteinLength: Glu21-Arg193
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus CD40, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.