Recombinant Cutibacterium acnes CAMP protein(Met1-Pro275), His-tagged

• Cat.No.: CAMP-3377C
• Product Overview: Recombinant Cutibacterium acnes CAMP factor (EPI1629568) (Met1-Tyr560) was expressed in Insect Cells with a polyhistidine tag at the C-terminus.

Specification

• Species: Cutibacterium acnes
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Pro275
• Form: Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Cutibacterium acnes CAMP factor consists of 264 amino acids and predicts a molecular mass of 34.13 kDa. It migrates as an approximately kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.