Recombinant COVID-19 Spike S2 ECD protein(Ser686-Pro1213), mFc-tagged

• Cat.No.: Spike-1224V
• Product Overview: Recombinant COVID-19 Spike Protein (S2 ECD) (YP_009724390.1) (Ser686-Pro1213) was expressed in Insect Cells with the Fc region of Mouse IgG1 at the C-terminus.

Specification

• Source: Insect Cells
• Species: COVID-19
• Tag: mFc
• Protein length: Ser686-Pro1213
• Form: Lyophilized from sterile 20mM Tris, 300mM NaCl, 100mM glycine, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S2 ECD, His tag) consists of 762 amino acids and predicts a molecular mass of 84.33 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: Spike
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2