Recombinant COVID-19 Spike S1 (N501Y, K417N, E484K) protein, His-tagged

• Cat.No.: Spike-5750V
• Product Overview: Recombinant COVID-19 Spike S1 (N501Y, K417N, E484K) protein(YP_009724390.1)(Val16-Arg685(N501Y, K417N, E484K)), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: C-His
• Protein length: Val16-Arg685(N501Y, K417N, E484K)
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized SARS-COV-2 Spike S1 (N501Y, K417N, E484K) , His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 75.9 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S spike protein [ Middle East respiratory syndrome-related coronavirus ]
• Official Symbol: S
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1
• Gene ID: 14254594
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2