Recombinant COVID-19 Spike RBD protein, hFc-tagged

• Cat.No.: Spike-231V
• Product Overview: Recombinant COVID-19 Spike RBD protein(QHD43416.1)(Arg319-Phe541), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: C-hFc
• Protein length: Arg319-Phe541
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus ACE2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 50.7 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: S spike protein [ Middle East respiratory syndrome-related coronavirus ]
• Official Symbol: S
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1
• Gene ID: 14254594
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2