Recombinant COVID-19 N protein(Met1-Ala419), His-tagged

• Cat.No.: N-1077V
• Product Overview: Recombinant SARS-CoV-2 Nucleocapsid Protein (YP_009724397.2) (Met1-Ala419) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Species: Sars-CoV-2
• Source: E.coli
• Tag: His
• Protein Length: Met1-Ala419
• Form: Lyophilized from sterile 50 mM PB, 500 mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant SARS-CoV-2 Nucleocapsid consists of 426 amino acids and predicts a molecular mass of 46.61 kDa.
• Purity: > 90 % as determined by SDS-PAGE. > 85 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.