Recombinant COVID-19 BJ.1 (Omicron) Spike S1+S2 trimer protein(Met1-Gln1208), His-tagged

Cat.No. : Spike-1241V
Product Overview : Recombinant SARS-CoV-2 (BJ.1) Spike S1+S2 (YP_009724390.1, with mutations T19I, del24-26, A27S, V83A, G142D, del145Y, H146Q, Q183E, V213E, G339H, R346T, L368I, S371F, D405N, R408S, K417N, N440K, V445P, G446S, S477N, T478K, V483A, E484A, F490V, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, G798D, F817P, A892P, A899P, A942P, Q954H, N969K, K986P, V987P, S1003I and furin cleavage site mutants) expressed in HEK293 with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant BJ.1).
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Source : HEK293
Species : COVID-19
Tag : His
Protein length : Met1-Gln1208
Form : Lyophilized from sterile 25mM Sodium Citrate, 200mM NaCl, 0.02%tween80, pH 6.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 BJ.1(Omicron) Spike S1+S2 trimer Protein(ECD, His Tag), the EC50 is 2.6-7.8 ng/mL.
Molecular Mass : The recombinant SARS-CoV-2 (BJ.1) Spike S1+S2 consists of 1227 amino acids and predicts a molecular mass of 136.37 kDa.
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
Official Symbol Spike
Synonyms Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1
Gene ID 43740568
Protein Refseq YP_009724390.1
UniProt ID P0DTC2

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