Recombinant COVID-19 3CLpro Protein, His-tagged

• Cat.No.: 3CLpro-82V
• Product Overview: Recombinant COVID-19 3CLpro Protein(YP_009725295.1) (Ser3264-Gln3569) was expressed in Insect Cells with a N-terminal polyhistidine tagged.

Specification

• Source: Insect Cells
• Species: COVID-19
• Tag: N-His
• Protein length: Ser3264-Gln3569
• Form: Lyophilized from sterile 20mM Tris, pH8.0, 300mM NaCl, 10% glycerol.
• Bio-activity: Measured by its ability to cleave a peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans.The specific activity is >5 pmols/min/μg.
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) 3CL Protease consists of 317 amino acids and predicts a molecular mass of 35.31 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.