Recombinant Chicken EPCAM protein, His-tagged

• Cat.No.: EPCAM-6765C
• Product Overview: Recombinant Chicken EPCAM aa. (Asp21~Gly260 linked with LEHHHHHH) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Chicken
• Source: E.coli
• Tag: His
• Protein Length: Asp21~Gly260 linked with LEHHHHHH
• Form: Freeze-dried powder
• Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 6.5.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: EPCAM epithelial cell adhesion molecule [ Gallus gallus (chicken) ]
• Official Symbol: EPCAM
• Synonyms: TACSTD1; ep-CAM; tumor-associated calcium signal transducer 1; epithelial cell adhesion molecule
• Gene ID: 421292
• mRNA Refseq: NM_001012564.1
• Protein Refseq: NP_001012582.1
• UniProt ID: Q5F381