Recombinant Cattle WNT3 Protein, His-tagged

• Cat.No.: WNT3-584C
• Product Overview: Recombinant Cattle WNT3 Protein (Val19-Lys355) with N-His tag was expressed in E. coli.

Specification

• Description: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.
• Source: E. coli
• Species: Cattle
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 41.4 kDa
  Accurate Molecular Mass: 41 kDa
• Protein length: Val19-Lys355
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: WNT3 Wnt family member 3 [ Bos taurus (cattle) ]
• Official Symbol: WNT3
• Synonyms: WNT3; Wnt family member 3; proto-oncogene Wnt-3; wingless-type MMTV integration site family member 3
• Gene ID: 540753
• mRNA Refseq: NM_001206024
• Protein Refseq: NP_001192953