Recombinant Canine TNFSF13B protein(Ala143-Leu292), hFc-tagged

• Cat.No.: TNFSF13B-1059C
• Product Overview: Recombinant Canine TNFSF13B (NP_001155182.1) (Ala143-Leu292) was expressed in HEK293 with the Fc region of Human IgG1 at the N-terminus.

Specification

• Species: Canine
• Source: HEK293
• Tag: Fc
• Protein Length: 143-292 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically 1-5 ng/mL.
• Molecular Mass: The recombinant canine TNFSF13B consists 412 amino acids and predicts a molecular mass of 45.5 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.