Recombinant Canine ERBB2 Protein, His-tagged, Alexa Fluor 647 conjugated

• Cat.No.: ERBB2-171CAF647
• Product Overview: A DNA sequence encoding the canine ERBB2 (ABW99108.1) (Met1-Thr652) (Alexa Fluor 647 conjugated) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: Dog
• Source: HEK293
• Tag: His
• Protein Length: Met1-Thr652, 641
• Form: Lyophilized
• Molecular Mass: The recombinant canine ERBB2 comprises 641 amino acids and has a predicted molecular mass of 70.4 kDa. The apparent molecular mass of the protein is approximately 75-95 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH7.4.

Gene Information

• Gene Name: ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Canis lupus familiaris ]
• Official Symbol: ERBB2
• Synonyms: ERBB2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); receptor tyrosine-protein kinase erbB-2; erbB-2; c-erbB-2; p185erbB2; proto-oncogene c-ErbB-2; HER-2
• Gene ID: 403883
• mRNA Refseq: NM_001003217
• Protein Refseq: NP_001003217

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.