Recombinant Canine CD40 Protein, LEVLFQ-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD40-153CAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Canine CD40 (Q7YRL5) (Met1-Ala194), fused with six amino acids (LEVLFQ) at the C-terminus, was produced in Human Cells.

Specification

• Species: Dog
• Source: HEK293
• Tag: LEVLFQ
• Protein Length: 181
• Form: Lyophilized
• Molecular Mass: The recombinant canine CD40 is a disulfide-linked homodimer. The reduced monomer comprises 181 amino acids and has a predicted molecular mass of 19.9 kDa. The apparent molecular mass of the protein is approximately 28-32 kDa in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Glu 21
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD40 centigrade D40 molecule, TNF receptor superfamily member 5 [ centigrade anis lupus familiaris(dog) ]
• Official Symbol: CD40
• Gene ID: 403469
• mRNA Refseq: NM_001002982
• Protein Refseq: NP_001002982
• UniProt ID: Q7YRL5