Active Recombinant Canine CA9 protein, hFc-tagged

• Cat.No.: CA9-1544C
• Product Overview: Recombinant Canine CA9 protein(Met1-Leu410), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Leu410
• Form: Lyophilized from sterile 25mM Tris, 0.15 M NaCl, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its esterase activity.The specific activity is > 10 pmoles/min/μg.
• Molecular Mass: The recombinant canine CA9/Fc is a disulfide-linked homodimer. The reduced monomer comprises 614 amino acids and has a predicted molecular mass of 67.6 kDa. The apparent molecular mass of the protein is approximately 79 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.