Recombinant Canine ACVR1, His-tagged

• Cat.No.: ACVR1-161C
• Product Overview: A DNA sequence encoding the canine ACVR1 (XP_549615.2) (Met1-Glu123) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: Canine
• Source: Human Cells
• Tag: His
• Protein Length: Met1-Glu123
• Description: Cytokines and hormones belonging to the transforming growth factor beta (TGFβ) superfamily such as the activin, anti-müllerian hormone (AMH), bone morphogenetic proteins (BMPs), and Nodal, are involved in a variety of physiological processes including growth, cell differentiation, homeostasis, osteogenesis, and apoptosis. They initiate signaling through heteromeric complexes of a type I and a type I I serine/threonine kinase receptor. The two type receptors are type I transmembrane proteins and are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found in the type I receptors. Type II receptors are required for binding ligands and recruitment of type I receptors which then form stable complexes, and type I receptors are essential for signaling transduction. ALK2, also termed as ACVR1, ActRI, SKR1, was initially identified as an activin type I receptor because of its ability to bind activin in concert with ActRII or IIB. In addition, ALK2 is also identified as a BMP type I receptor. ACVR1 has been demonstrated to form complex with either the BMP-2/7- bound BMPR-II or ACVR2A/ACVR2B and transduce signals to downstream components R-SMADs. Recombinant soluble ALK2 does not bind activin. Mutations in this gene are associated with fibrodysplasia ossificans progressive.
• Form: Lyophilized from sterile PBS, pH7.4.
• Molecular Mass: The recombinant canine ACVR1 comprises 114 amino acids and has a predicted molecular mass of 13 kDa. The apparent molecular mass of the protein is approximately 19 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: ACVR1 activin A receptor, type I [ Canis lupus familiaris ]
• Official Symbol: ACVR1
• Synonyms: ACVR1; activin A receptor, type I; activin receptor type-1
• Gene ID: 478757
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; TGF-beta signaling pathway, organism-specific biosystem; TGF-beta signaling pathway, conserved biosystem