Recombinant Bovine transforming growth factor beta 3 Protein, His tagged

• Cat.No.: TGFB3-32B
• Product Overview: DNA sequence encoding Bovine TGFB3 sp (A6QP91, 341-452aa) including a C terminal His tag was expressed in HEK293 cells.

Specification

• Species: Bovine
• Source: HEK293
• Tag: His
• ProteinLength: 341-452aa
• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. This protein is involved in embryogenesis and cell differentiation, and may play a role in wound healing. Mutations in this gene are a cause of aortic aneurysms and dissections, as well as familial arrhythmogenic right ventricular dysplasia 1.
• Tag: C-His
• Bio-activity: The ED(50) was determined by the ability to inhibit the proliferation of mouse HT-2 cells 0.05-0.1 ng/mL, corresponding to a specific activity of 2×10^7 units/mg.
• Molecular Mass: 13-15 kDa
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg (1 EU/μg).
• Purity: > 95%, as determined by SDS-PAGE and HPLC
• Usage: This product is for research purposes only. It may not be used for therapeutics or diagnostic purposes.
• Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 centigrade.
• Storage Buffer: Lyophilized from 0.2 μm filtered PBS solution pH7.4
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Gene Information

• Gene Name: TGFB3 transforming growth factor beta 3 [ Bos taurus (cattle) ]
• Official Symbol: TGFB3
• Synonyms: TGFB3; transforming growth factor beta 3; transforming growth factor beta-3 proprotein
• Gene ID: 538957
• mRNA Refseq: NM_001101183
• Protein Refseq: NP_001094653
• UniProt ID: A6QP91