Recombinant Bovine Tgfb1 Protein, Ala279-Ser390

• Cat.No.: Tgfb1-33B
• Product Overview: Recombinant Bovine Tgfb1 Protein (Ala279-Ser390) without tag was expressed in CHO.

Specification

• Description: TGF-β1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-β1 superfamily that share a characteristic cystine knot structure. TGF-β1, -2 and -3 are highly pleiotropic cytokines that act as cellular switches to regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-β isoform has some non-redundant function; for TGF-β1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation and died of overwhelming inflammation. TGF-β1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-β RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-β RI (also called activin receptor‑like kinase (ALK)-5), or alternatively, ALK-1. This complex phosphorylates and activates Smad proteins that regulate transcription.
• Source: CHO
• Species: Bovine
• Protein length: Ala279-Ser390
• Bio-activity: ED50 < 0.2 ng/ml, measured in ability to inhibit the mouse IL-4-dependent proliferation of HT-2 cells.
• Molecular Mass: Theoretical Molecular Weight: 12.8 kDa (monomer)
  Apparent Molecular Weight: ~12 kDa, on SDS-PAGE under reducing conditions.
• Endotoxin: < 0.2 EU/μg of protein by gel clotting method
• Purity: ≥ 95% as analyzed by SDS-PAGE
• Storage: Upon receiving, this product remains stable for up to 6 months at lower than -70 centigrade. Upon reconstitution, the product should be stable for up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. For long-term storage after reconstitution, it is recommended that a carrier protein (e.g., 0.1% BSA) be added. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 centigrade.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in 50 mM NaAc, 50 mM NaCl, pH 5.0.
• Reconstitution: It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O or 50 mM Citrate up to 100 μg/mL.

Gene Information

• Gene Name: TGFB1 transforming growth factor beta 1 [ Bos taurus (cattle) ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor beta 1; transforming growth factor beta-1 proprotein; TGF-beta-1; transforming growth factor, beta 1 (Camurati-Engelmann disease)
• Gene ID: 282089
• mRNA Refseq: NM_001166068
• Protein Refseq: NP_001159540
• UniProt ID: P18341