Recombinant Bovine Fibroblast Growth Factor 2

• Cat.No.: FGF2-650B
• Product Overview: Recombinantbovine FGF2 is a single, non-glycosylated polypeptide chain containing 172amino acids.

Specification

• Species: Bovine
• Source: Bovine
• Tag: Non
• Description: FGF2 is a single-chainpolypeptide growth factor that plays a significant role in the process ofwound healing and is a potent inducer of angiogenesis. Several different formsof the human protein exist ranging from 18-24 kDa in size due to the use of alternativestart sites within the FGF2 gene.
• Form: Lyophilized from asterile filtered solution in PBS, pH 7.4 + 4% mannitol.
• Purity: >97% by SDS-PAGEand HPLC analyses.
• Sequence: MTMITNSSSVPGDPLESMAA GSITTLPALP EDGGSGAFPP GHFKDPKRLY CKNGGFFLRI HPDGRVDGVR EKSDPHIKLQLQAEERGVVS IKGVCANRYL AMKEDGRLLA SKCVTDECFF FERLESNNYN TYRSRKYSSW YVALKRTGQY KLGPKTGPGQKAILFLPMSA KS
• Molecular Weight: 18.5 kDa
• Biological Activity: Calculated by the dose dependantproliferation of BALB/c 3T3 cells, the ED50 is < 1.0 ng/ml.
• Endotoxin: <1 ng/μg of FGF2
• Reconstitution: Centrifugevial prior to opening. Add sterile distilled water or aqueousbuffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be madein appropriate buffered solutions.
• Storage: Stable at2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to1 week at 2-8°C. For longer term, store in working aliquots below -20°C.Avoid repeated freeze/thaw cycles.
• OfficialSymbol: FGF2
• Pathways: MAPKsignaling pathway; Melanoma; Pathways in cancer; Regulation of actincytoskeleton

Gene Information

• Gene Name: FGF2 fibroblastgrowth factor 2 (basic) [ Bos taurus ]
• Synonyms: FGF2; fibroblast growthfactor 2 (basic); heparin-binding growth factor 2; BFGF; HBGF-2;basic fibroblast growth factor; Heparin-binding growth factor 2; Basicfibroblast growth factor; bFGF; FGF-2
• Gene ID: 281161
• mRNA Refseq: NM_174056
• Protein Refseq: NP_776481
• UniProt ID: P03969
• Chromosome Location: 17
• Function: growth factor activity; heparinbinding; protein binding; voltage-gated calcium channel activity