Recombinant Borrelia burgdorferi sensu stricto (B31) ErpD/Arp37 Protein, MBP-tagged

• Cat.No.: BOR-030
• Product Overview: Recombinant Borrelia burgdorferi ErpD protein, fused to an MBP-tag and produced in E. coli (>90% purity).

Specification

• Tag: MBP
• Background: ErpD (OspE/F-Related Protein D), also known as Arp37, is encoded by the spirochete B. burgdorferi, which is carried by Ixodes ticks. Strain B31 is the type strain (ATCC 35210) for this organism and was derived by limited dilutional cloning from the original Lyme-disease tick isolate derived by A. Barbour (Johnson, et al., 1984).
  Several studies have demonstrated that infected humans and animals produce antibodies directed against Erp proteins within the first 2-4 weeks of infection, suggesting Erp synthesis occurs during the initial stages of vertebrate infection. Surface-exposed Erp proteins may then facilitate interactions with host tissues during the establishment of vertebrate infection (Fraser, et al., 1997).
  Erp proteins from B. burgdorferi are believed to be lipoproteins, based on their predicted amino acid sequences. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host (Stevenson, et al., 1996).
• Purity: >90% by SDS-PAGE
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
• Stability: At +4 centigrade: Not determined.
  At -80 centigrade: Not determined.
• Freezing: Can be frozen, but avoid multiple freeze/thaw cycles.
• Storage: Short Term Storage: +2 centigrade to +8 centigrade
  Long Term Storage: -80 centigrade
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Notes: This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations.
• Type: Recombinant
• ClassID 1: Infectious Disease

Gene Information

• Synonyms: Borrelia burgdorferi sensu stricto (B31) ErpD/Arp37