Recombinant Active E. coli argG Protein (1-447), N-6×His-tagged

• Cat.No.: argG-12E
• Product Overview: Recombinant Active E. coli argG Protein with N-6×His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
• Source: E. coli
• Species: E. coli
• Tag: His
• Form: Lyophilized
• Bio-activity: The specific activity is 500 mU/mg based on its ability to convert citrulline to argininosuccinate.
• Molecular Mass: 50 kDa including tags
• Protein length: 1-447 (Full length)
• Purity: >= 95% SDS-PAGE
• Applications: SDS-PAGE, Functional Studies
• Storage Buffer: 0.38% Potassium phosphate, 0.46% Sodium chloride, 0.06% Tris HCl, 0.59% Potassium chloride, 0.018% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.07% EDTA, 0.107% Magnesium acetate, 1.2% BSA, 32% Trehalose, 9.6% Dextran, 1% Alcohol Ethoxylate
• Reconstitution: Reconstitute the lyophilized protein in H2O with concentration of 1.0 mU/mL. Incubate the reconstituted protein at 25 centigrade for 15 minutes.
• Shipping: Shipped at 4 centigrade. Store at -20 centigrade. Avoid freeze/thaw cycle.

Gene Information

• Gene Name: argG argininosuccinate synthetase [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: argG
• Synonyms: argG; argininosuccinate synthetase; ECK3161
• Gene ID: 947590
• Protein Refseq: NP_417640
• UniProt ID: P0A6E4