Active Recombinant COVID-19 Spike protein RBD(N501Y), His-tagged

• Cat.No.: Spike-145V
• Product Overview: A DNA sequence encoding the COVID-19 Spike RBD(N501Y)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(N501Y)) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: COVID-19
• Source: HEK293
• Tag: His
• Protein Length: Arg319-Phe541(N501Y)
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (mFc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His is 3-20 ng/mL.
  2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein with an affinity constant of 0.827 nM as determined by Octet RED96e system.
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Adaptation of a Model Spike Aptamer for Isothermal Amplification-Based Sensing (2023)