Recombiant Hmuan AlkB, Alkylation Repair Homolog 2, His-tagged

• Cat.No.: ALKBH2-977H
• Product Overview: Recombinant human ALKBH2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: ALKBH2-977H
• Description: Alpha-ketoglutarate-dependent dioxygenasealkB homolog 2, also known ALKBH2, belongs to the ALKB family. Expressed in heart, colon, liver, testis, ovary, prostate and small intestine, ALKBH2 uses iron as a cofactor and functions as a dioxygenase that catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and α-ketoglutarate for enzymatic activity.
• Formulation: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol
• Molecular Weight: 31.4kDa (281aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS – PAGE
• Sequences of amino acids: MGSSHHHHH HSSGLVPRGSHMDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K
• concentration: 0.5 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ALKBH2 alkB, alkylation repair homolog 2 (E. coli) [ Homo sapiens ]
• Synonyms: ALKBH2;alkB, alkylation repair homolog 2 (E. coli);ABH2; FLJ99103; MGC90512; alpha-ketoglutarate-dependent dioxygenasealkB homolog 2;oxy DC1;2OG-Fe(II) oxy DC1;alkylated DNA repair protein alkB homolog 2;EC 1.14.11.
• Gene ID: 121642
• MIM: 610602
• mRNA Refseq: NM_001001655
• Protein Refseq: NP_001001655
• UniProt ID: Q6NS38
• Chromosome Location: 12q24.11
• Function: 2-oxoglutarate-dependent dioxygenase activity;DNA-N1-methyladenine dioxygenase activity;cytosine C-5 DNA demethylase activity;damaged DNA binding;ferrous iron binding;metal ion binding;oxidoreductase activity;oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen.