Rabbit Monoclonal Antibody to Mouse Lymphocyte Antigen 86

• Cat.No.: CAB11581RM
• Product Overview: Rabbit Monoclonal Antibody to Mouse Lymphocyte Antigen 86

Specification

• Species: Mouse
• Source: Rabbit
• Tag: Non
• Antigen Description: MD-1 and MD-2 are secretory glycoproteins that exist on the cell surface in complexes with transmembrane proteins. MD-1 is anchored by radioprotective 105 (RP105) which is a molecule containing leucine-rich repeats and is expressed on B cells, dentritic cells and macrophages, while MD-2 is associated with TLR4. MD-1 is required for efficient RP105 cell surface expression and function. It is indicated that the RP105/MD1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells, and also plays a role in protecting against apoptosis, B-cell proliferation, etc. Mouse MD-1 cDNA encodes a 162 amino acid precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 40% and 66% amino acid sequence identity with chicken and human MD-1 respectively. MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney.
• Specificity: Mouse MD-1 / LY86. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
• Immunogen: Recombinant Mouse MD-1 / LY86 Protein
• Isotype: Rabbit IgG
• Clone: A417
• Applications: Western blot; ELISA
• Dilution: Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse LY86 in WB. Using a DAB detection system, the detection limit for Mouse LY86 is approximately 0.5 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse LY86. The detection limit for Mouse LY86 is approximately 0.0195 ng/well.
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Mouse MD-1 / LY86
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Ly86 lymphocyte antigen 86 [ Mus musculus ]
• Official Symbol: LY86
• Synonyms: LY86; lymphocyte antigen 86; ly-86; protein MD-1; MD1; MD-1
• Gene ID: 17084
• mRNA Refseq: NM_010745
• Protein Refseq: NP_034875