Rabbit Monoclonal Antibody to Human Serine Peptidase Inhibitor, Clade F, Member 1

• Cat.No.: CAB11602RH
• Product Overview: Rabbit Monoclonal Antibody to Human Serine Peptidase Inhibitor, Clade F, Member 1

Specification

• Antigen Description: Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein which has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression.
• Specificity: Human PEDF / SerpinF1. No cross-reactivity in ELISA with Mouse PEDF / SerpinF1; Human cell lysate (293 cell line)
• Immunogen: Recombinant Human PEDF / SerpinF1 Protein
• Host: Rabbit
• Isotype: Rabbit IgG
• Species: Human
• Clone: A014
• Applications: Western blot; ELISA
• Dilution: Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human SERPINF1 in WB.Using a DAB detection system, the detection limit for Human SERPINF1 is approximately 0.5 ng/lane under non-reducing conditions and 0.25 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SERPINF1. The detection limit for Human SERPINF1 is approximately 0.00975 ng/well.
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human PEDF / SerpinF1
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: SERPINF1 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 [ Homo sapiens ]
• Official Symbol: SERPINF1
• Synonyms: SERPINF1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; PEDF,serine (or cysteine) proteinase inhibitor, clade F (alpha 2 antiplasmin, pigment epithelium derived factor), member 1; pigment epithelium-derived factor; EPC 1; PIG35; pigment epithelium derived factor; proliferation inducing protein 35; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; OI6; OI12; PEDF; EPC-1
• Gene ID: 5176
• mRNA Refseq: NM_002615
• Protein Refseq: NP_002606
• MIM: 172860
• UniProt ID: P36955
• Chromosome Location: 17p13.3
• Function: serine-type endopeptidase inhibitor activity