Rabbit Monoclonal Antibody to Human Nephroblastoma Overexpressed

• Cat.No.: CAB11588RH
• Product Overview: Rabbit Monoclonal Antibody to Human Nephroblastoma Overexpressed

Specification

• Antigen Description: Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is secreted protein which belongs to the?CCN family. CCN3 expression was observed in a broad variety of tissues from the early stage of development. It contains one?CTCK (C-terminal cystine knot-like) domain, one?IGFBP N-terminal domain, one?TSP type-1 domain, and one?VWFC domain. CCN3, a founding member of the CCN family of growth regulators, was linked with hematology in 2003 when it was detected in human serum. CCN3 acts through the core stem cell signalling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of a number of cell lineages including hematopoietic, osteogenic and chondrogenic. Altered CCN3 expression is associated with numerous solid tumors including glioblastoma, melanoma, adrenocortical tumours, prostate cancer and bone malignancies including osteosarcoma. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis and a crucial component within the bone marrow microenvironment.
• Specificity: Human CCN3 / NOV / IGFBP9. No cross-reactivity in ELISA with Human WISP1; Human cell lysate (293 cell line)
• Immunogen: Recombinant Human CCN3 / NOV / IGFBP9 Protein
• Host: Rabbit
• Isotype: Rabbit IgG
• Species: Human
• Clone: A131
• Applications: Western blot; ELISA
• Dilution: Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human NOV in WB.Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human NOV. The detection limit for Human NOV is approximately 0.00975 ng/well.
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human CCN3 / NOV / IGFBP9
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: NOV nephroblastoma overexpressed [ Homo sapiens ]
• Official Symbol: NOV
• Synonyms: NOV; nephroblastoma overexpressed; nephroblastoma overexpressed gene; protein NOV homolog; CCN3; IGFBP9; CCN family member 3; IGF-binding protein 9; insulin-like growth factor-binding protein 9; nephroblastoma-overexpressed gene protein homolog; NOVh; IBP-9; IGFBP-9
• Gene ID: 4856
• mRNA Refseq: NM_002514
• Protein Refseq: NP_002505
• MIM: 164958
• UniProt ID: P48745
• Chromosome Location: 8q24.12
• Pathway: Delta-Notch Signaling Pathway, organism-specific biosystem
• Function: growth factor activity; insulin-like growth factor binding