Rabbit Anti-Pasteurella Multocida Toxin Antibody

• Cat.No.: PAS-534
• Product Overview: Anti-Pasteurella mulcosida toxin antibody, rabbit polyclonal.

Specification

• Background: Pasteurella multocida is a frequent commensal of the respiratory tract of animals. As a facultative pathogen, it can lead to severe and economically important diseases, such as shipping fever in cattle, snuffles in rabbits, and fowl cholera in poultry and in humans is a zoonotic disease caused by scratches, bites, and saliva from pet animals such as cats and dogs (Harper et al., 2006; Wilkie et al., 2012).
  P. multocida is a gram-negative coccobacillus. It is a non-motile, facultative anaerobe, and is generally penicillin-sensitive. There are five commonly isolated serogroups (A, B, D, E, and F), which are classified by the composition of the polysaccharide capsule. Most human infections are caused by serogroups A and D. P. multocida is the most common cause of soft tissue infection in humans following bites or scratches from dogs and cats. Pasteurella species are part of the normal flora of the mouth and upper respiratory tract of many animals, both wild and domestic. The animals with the highest rate of carriage are cats; dogs have the second-highest rate of carriage. One of the major virulence factors of P. multocida is the protein toxin PMT (P. multocida toxin), which is produced by serogroup D and some A strains. PMT is the causative agent to induce atrophic rhinitis in pigs, characterized by shortening and twisting of the snout due to the loss of nasal turbinate bones (Horiguchi, 2012).
  PMT activates various heterotrimeric G proteins and many are substrates of the toxin. The 146- kDa toxin PMT comprises 1,285 amino acids (aa) (Orth & Aktories, 2012). Different domains of PMT are involved in cell uptake and intracellular action. The receptor binding and translocation domains are located in the N terminus (aa 1 to 574). Whereas the receptor binding domain has not been characterized in detail, two amphipathic helices, covering residues 402 to 457, are suggested to be involved in membrane insertion and translocation (Baldwin et al., 2004). So far, the cell surface receptor of PMT is not known (Brothers et al., 2011). PMT can be utilized as a transporter to carry a non-cell-permeating protein (DTa) as a fusion protein into the cytosol of host cells (Bergmann et al., 2013).
• Formulation: PBS, 50% glycerol.
• Applications: Applications include Western blotting (1/500-1/1,000 dilution), Dot blot (assay dependent), ELISA (assay dependent).
• ClassID 1: Infectious Disease

Gene Information

• Synonyms: Rabbit Anti-Pasteurella Multocida Toxin Antibody