Polymyxin B Agarose

• Cat.No.: Polymyxin B-1A
• Product Overview: The polymyxin B is intended for use in low, medium, or high pressure chromatographic applications. The support consists of polymeric macroporous (1,000 ?) 45μm beads with USP Grade polymyxin B covalently attached. The main application of this product is the removal of endotoxin molecules from biological and aqueous solutions by affinity chromatography. For best performance, this product should be used in dust-freeGMP-grade facilities, all buffers and solutions should be prepared with pyrogen-free water, and, after use, the matrix should be sanitized before use again by treatment with 0.1 N NaOH. Contact time with 0.1 N NaOH should be minimized. Chromatography can beperformed at room temperature or at 4°C.

Specification

• Tag: Non
• Cat.no: Polymyxin B-1A
• Form: aqueous glycerol suspension Supplied as a 50% suspension
• Matrix: Cross-linked 4% beaded agarose
• Functional group: Polymyxin B
• Average particle size: 52 – 165 μm
• Dynamic binding capacity: 200-500 μg/mL binding capacity (lipopolysaccharide)
• Recommended flow rate: 2,000 cm/hr
• Chemical stability: High chemical stability (withstands sanitization with 0.1 M NaOH)
• pH CIP range: 3-10