Phosphoric acid transacetylase (PTA) activity assay kit

• Cat.No.: KITZ-071
• Product Overview: Phosphoric acid transacetylase (PTA) is one of the key enzymes related to acetic acid metabolism. (PTA) catalyzes the reaction of coenzyme A and acetyl phosphate to generate acetyl coenzyme A and inorganic phosphorus. The increase in inorganic phosphorus is measured by the ammonium molybdate phosphorus determination method to determine the activity of PTA enzyme.

Specification

• Size: 24 Samples
• Storage: -20°C
• Shipping: Ice pack
• Kit Components: Extraction Liquid: Liquid, 30mL × 1 bottle, store at 4°C.
  Reagent One: Liquid, 20mL × 1 bottle, store at 4°C.
  Reagent Two: Powder, mg × 1 vial, store at 4°C. Before use, shake to bring the reagent to the bottom, then add 1.1mL of distilled water and mix well to dissolve and reserve.
  Reagent Three: Powder, mg × 1 vial, store at -20°C. Before use, shake to bring the reagent to the bottom, then add 0.55mL of distilled water and mix well to dissolve and reserve.
  Reagent Four: Liquid, 4mL × 1 bottle, store at 4°C.
  Reagent Five: Consists of A and B parts A: Powder, mg × 1 bottle B: Liquid, 3mL × 1 bottle Store at 4°C. Before use, add 2.9mL of B liquid, then add 37.1mL of distilled water, mix well to dissolve and reserve.
  Standard: Powder, mg × 1 vial, store at 4°C. This reagent is used if recalibration of the standard curve is necessary.
• Materials Required but Not Supplied: Visible spectrophotometer, 1mL glass cuvettes (optical path 1cm), benchtop centrifuge, adjustable pipette, mortar, ice, and distilled water.