Phosphatidylcholine Assay Kit

• Cat.No.: Kit-0662
• Product Overview: Phosphatidylcholine Assay Kit is used for measuring phosphatidylcholine.

Specification

• Tag: Non
• Description: Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid. The Phosphatidylcholine Assay Kit is a simple convenient means of measuring Phosphatidylcholine in a variety of biological samples. The kit utilizes an enzyme-coupled assay in which PC is hydrolyzed, releasing choline which is subsequently oxidized resulting in development of the OxiRed probe to generate fluorescence (Ex/Em = 535/587 nm) and absorbance (570 nm). The kit measures PC in the range of 0.1 to 10 nmol per sample. PC is present in serum at ~ 0.2-2.5 mM (~50-200 mg/dL).
• Target Species: Mammals
• Usage: For research use only (RUO)
• Storage: Store the kit at -20°C, protect from light. Allow Assay Buffer to warm to room temperature before use. Briefly centrifuge vials prior to opening. Read the entire protocol before performing the assay.
• Kit Components: PC Assay Buffer 25 mLOxiRed Probe 0.2 mLPC Hydrolysis Enzyme, lyophilize. 1 vialPC Development Mix, lyophilize. 1 vialPC Standard (10 μmol), lyophilize. 1 vial
• Detection method: Colorimetric, Fluorometric
• Compatible Sample Types: Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine