OncoStat Panel

• Cat.No.: NUC-063
• Product Overview: A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli.

Specification

• Source: E.coli
• Tag: Non
• Description: A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243*; H4-P62805) wrapped by 147 base pairs of barcoded Widom 601 positioning sequence DNA. The OncoStat panel is comprised of a pool of 1 unmodified (H3.3) plus 7 histone H3.3 point mutations: H3.3K4M, H3.3K9M, H3.3K27M, H3.3G34R, H3.3G34V, H3.3G34W, H3.3K36M. Each distinctly modified nucleosome is distinguishable by a unique sequence of DNA (“barcode”) at the 3’ end that can be deciphered by qPCR or next-generation sequencing. Each of the 8 nucleosomes in the pool is wrapped by 2 DNA species, each containing a distinct barcode (”A” and “B”, see SNAP-ChIP Manual) allowing for an internal technical replicate.
• Form: Purified recombinant mononucleosomes, containing a mixture of 16 (1 unmodified plus 15 unique) H3 and H4 Purified recombinant mononucleosomes, containing a mixtureof 8 (1 unmodified plus 7 point mutations) nucleosomes in 10mM sodium cacodylate, pH 7.5, 100 mM NaCl, 1 mM EDTA,50% glycerol (w/v), 1x Protease Inhibitor cocktail, 100 ug/mLBSA, 10 mM β-mercaptoethanol. Molarity = 0.6 nM.
• Molecular Mass: ~199283.7 Da (average MW of all 8 nucleosomes)
• Applications: SNAP-ChIP OncoStats are highly purified recombinantmononucleosomes and are suitable for use as spike-in controlsfor ChIP reactions, for antibody specificity testing or for effectorprotein binding experiments. See manual for more information.
• Storage: Stable for six months at -20°C from date of receipt.