Non-cleavable Recombinant Human Tpa

• Cat.No.: PLAT -79H
• Product Overview: The addition of an arginine to glutamic acid mutation generates human tPA that is 100percent single chain and resistant to cleavage by plasmin. The mutation site is R275E on the mature protein and R310E on the complete mRNA sequence (UniProtKB: locus TPA_HUMAN, accession P00750). Recombinantly produced in insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Form: Frozen liquid
• Molecular Mass: 70,000
• Purity: >95% by SDS-PAGE analysis
• Usage: For in vitro laboratory use only
• Notes: Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
• Storage: -70°C
• Concentration: 2.0 mg/ml
• Storage Buffer: 0.4M HEPES; 0.1M NaCl; pH 7.4
• Shipping: Dry ice

Gene Information

• Gene Name: PLAT plasminogen activator, tissue [ Homo sapiens ]
• Official Symbol: PLAT
• Synonyms: PLAT; plasminogen activator, tissue; tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA); TPA; T-PA; DKFZp686I03148
• Gene ID: 5327
• mRNA Refseq: NM_000930
• Protein Refseq: NP_000921
• MIM: 173370
• UniProt ID: P00750
• Chromosome Location: 8p11.21
• Pathway: Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Dissolution of Fibrin Clot, organism-specific biosystem; Endochondral Ossification, organism-specific biosystem; Hemostasis, organism-specific biosystem
• Function: peptidase activity; protein binding; serine-type endopeptidase activity; serine-type endopeptidase activity