Ni-NTA Polar IMAC PMMA resin

• Cat.No.: IMAC-59P
• Product Overview: Ni-NTA Polar IMAC PMMA resin packing is a metal-affinity packing suitable for purifying recombinant proteins with histidine (His) tags, which are widely used in the biotechnology field. The filler is based on hydrophilic polymethacrylate microspheres with A particle size of 30 μm or 60 μm and a pore size of 800 Å, and has high physical and chemical stability. The surface of the filler has a high degree of hydrophilicity, which avoids non-specific adsorption with biological samples to the greatest extent. On the hydrophilic surface, carboxylic acid groups that can chelate polyvalent metal ions are linked with a proprietary connector.

Specification

• Form: 50% (v/v) suspension, preserved in 20% ethanol
• Matrix: Hydrophilic polymethacrylate
• Average particle size: 30 μm; 60 μm
• Ligand: NTA
• pH stability range: Base filler Polar MC-CA Excel: 1-13; Metal ion chelating filler Polar MC-Ni Excel: 1-13
• Capacity: Polar MC30-CA Excel >120 μeq. carboxylic group /mL
  Polar MC30-Ni Excel 40-60 μeq.Ni++ /mL
  Polar MC60-CA Excel >100 μeq. carboxylic group /mL
  Polar MC60-Ni Excel 40-60 μeq.Ni++ /Ml
• Histidine labeled protein (HIStidine protein) loading: Polar MC30-Ni Excel, ~20 mg/mL
  Polar MC60-Ni Excel, ~15 mg/mL
• Operating Temperature: ≤40°C
• Operating pressure: ≤30 MPa (30 bar)
• Mobile phase compatibility: Aqueous solution, or a mixture of water and acetonitrile, acetone, or methanol.
  Commonly used buffers: phosphate, trimethylol aminomethane (Tris) and acetate, also compatible with 6M guanidine hydrochloride, 8M urea, 0.1 M EDTA, 10 mM DTT
• Linear flow rate: ≤1800 cm/h
• Storage: Store at 20% ethanol, 4-25°C.