Ni Agarose high flow

• Cat.No.: Ni-001A
• Product Overview: Ni Agarose is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins.

Specification

• Tag: Non
• Cat.no: Ni-001A
• Characteristics: Ni Agarose High Flow charged with nickel ions will selectively retain proteins containing a histidine-tag. Histidinetagged protein is then eluted using buffers containing imidazole. For purification of insoluble histidine-tagged proteins (fusion proteins expressed as inclusion bodies) requiring denaturing conditions, up to 8 M urea or 6 M guanidine hydrochloride can be used in the buffers to solubilise insoluble proteins.
• Size: 10 mL, 25 mL, 500 mL
• Matrix: 4% cross-linked agarose
• Average particle size: 90 μm
• Ligand: Ni2+
• Dynamic binding capacity: 20 mg of 6xHis-tagged protein (27 kDa) /ml settled resin
• Recommended column height: 5‐20cm
• Temperature Stability: 4‐40 °C