Native Jackbean Concanavalin A Lectin (Con A), FITC-Conjugated

• Cat.No.: Con A-300J
• Product Overview: Native Jackbean Concanavalin A Lectin (Con A) - FITC (Fluorescein)
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: α-Mannose, α-Glucose
  Inhibiting or Eluting Sugar: α-Methylmannoside + α-Methylglucoside
  Divalent Ions: Ca++, Mn++
  Excitation: 495 nm
  Emission: 515 nm
  Mitogenic Activity: Yes
  Hazardous Shipping: Non-hazardous
  Fluorescence: Green

Specification

• Species: Jack Bean
• Source: Jackbean
• Description: Affinity purified concanavalin A (Con A) is composed of two identical subunits of 237 amino acid residues (MW: 26,000 without any cystine residues). Both ions are required for binding with optimum activity at a pH 7. 0. Con A does not have a blood group specificity. It exhibits mitogenic activity with lymphocytes and cancer cells. While lymphocytes and cancer aggregate by Con A; normal white cells do not. Normal cells react to Con A after proteolytic treatment which suggests that trypsinization causes clustering of the reactive glycan residues on the membrane. Con A interacts with cell types include locust muscle fibers, adipocytes, and rat liver plasma membrane components. This lectin induces endoreduplication in mammalian cells and it reacts with E. coli, Dictyostelium discoideum and Bacillus lipopolysaccharides. Immobilized Con A has been used in affinity chromatography purifications of a wide variety of glycoproteins and cellular structures. Concanavalin A (Jackbean) Lectin (Con A) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications.
• Form: Liquid
• Purity: High Purity Grade
• Stability: Shelf Life: 1 year
• Storage: At 2-8 centigrade.
• Concentration: 5 mg/mL

Gene Information

• Official Symbol: Con A
• Synonyms: Concanavalin A Lectin; Con A