Native Jackbean Concanavalin A Lectin (Con A), Ferritin-Conjugated

• Cat.No.: Con A-258J
• Product Overview: Native Jackbean Concanavalin A Lectin (Con A) - Ferritin
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: α-Mannose, α-Glucose
  Inhibiting or Eluting Sugar: α-Methylmannoside + α-Methylglucoside
  Divalent Ions: Ca++, Mn++
  Mitogenic Activity: Yes
  Hazardous Shipping: Non-hazardous

Specification

• Species: Jack Bean
• Source: Jackbean
• Description: Affinity purified concanavalin A (Con A) is composed of two identical subunits of 237 amino acid residues (MW: 26,000 without any cystine residues). Con A does not have a blood group specificity. It exhibits mitogenic activity with lymphocytes and cancer cells. While lymphocytes and cancer aggregate by Con A; normal white cells do not. Normal cells react to Con A after proteolytic treatment which suggests that trypsinization causes clustering of the reactive glycan residues on the membrane. Con A interacts with cell types include locust muscle fibers, adipocytes, and rat liver plasma membrane components. This lectin induces endoreduplication in mammalian cells and it reacts with E. coli, Dictyostelium discoideum and Bacillus lipopolysaccharides. Immobilized Con A has been used in affinity chromatography purifications of a wide variety of glycoproteins and cellular structures. Concanavalin A (Jackbean) Lectin (Con A) is conjugated to ferritin, that has a molecular weight of 474,000 g/mol (all 24 subunits combined). Ferritin-conjugated lectins can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins. These conjugates can be viewed via electron microscopy.
• Form: Liquid
• Purity: High Purity Grade
• Stability: Shelf Life: 1 year
• Storage: At 2-8 centigrade.
• Concentration: 1 mg/mL

Gene Information

• Official Symbol: Con A
• Synonyms: Concanavalin A Lectin; Con A